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Efficiencies of recovery and extraction of trace DNA from non-porous surfaces

Wood, I; Park, S; Tooke, J; Smith, O; Morgan, RM; Meakin, GE; (2017) Efficiencies of recovery and extraction of trace DNA from non-porous surfaces. Forensic Science International: Genetics Supplement Series , 6 e153-e155. 10.1016/j.fsigss.2017.09.022. Green open access

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Abstract

DNA recovery and extraction efficiencies are key considerations for trace DNA interpretation in casework, but prior studies have tended to focus on assessing these for body fluids rather than trace DNA. This study therefore examined the recovery and extraction of trace DNA using different collection methods from a range of non-porous surfaces relevant to crimes including homicides, terror attacks, and wildlife poaching. Direct extraction of DNA from solutions of a known concentration revealed absolute extraction efficiencies of ∼82%. When DNA was extracted from swabs seeded with the DNA solution, a similarly high efficiency of ∼85% was achieved from nylon-flocked swabs, with a lower efficiency of ∼55% from cotton swabs. However, when DNA was recovered from non-porous surfaces with swabs, ∼55% of DNA was still recovered from plastic knife handles, but lower efficiencies were achieved from the other substrates, particularly metal cable. Varied and poor recovery was observed using mini-tapes and requires further investigation. These results demonstrate that > 50% recovery efficiency of trace DNA is achievable with both swab types, although recovery rates may be affected by surface type and/or practitioner experience.

Type: Article
Title: Efficiencies of recovery and extraction of trace DNA from non-porous surfaces
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.fsigss.2017.09.022
Publisher version: http://doi.org/10.1016/j.fsigss.2017.09.022
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Touch DNA, Trace DNA, Extraction efficiency, Recovery efficiency
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Security and Crime Science
URI: https://discovery.ucl.ac.uk/id/eprint/1576329
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