UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis

Helliwell, JA; Thomas, DS; Papathanasiou, V; Homer-Vanniasinkam, S; Desai, A; Jennings, LM; Rooney, P; ... Ingham, E; + view all (2017) Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis. Journal of Tissue Engineering , 8 pp. 1-12. 10.1177/2041731417724011. Green open access

[thumbnail of Homer-Vanniasinkam_2041731417724011.pdf]
Preview
Text
Homer-Vanniasinkam_2041731417724011.pdf - Published version

Download (1MB) | Preview

Abstract

The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment. Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests. There was no microscopic evidence of cells in decellularised porcine dermis. DNA content was reduced by 98.2% compared to cellular porcine skin. There were no significant differences in the biomechanical parameters studied or evidence of cytotoxicity. The decellularised porcine dermis retained residual alpha-gal epitope. Basement membrane collagen IV immunostaining was lost following decellularisation; however, laminin staining was retained.

Type: Article
Title: Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1177/2041731417724011
Publisher version: http://doi.org/10.1177/2041731417724011
Language: English
Additional information: Copyright © The Author(s) 2017. Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Keywords: Dermis, decellularisation, skin, xenograft
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1575398
Downloads since deposit
47Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item