Fisusi, FA;
Notman, R;
Granger, LA;
Malkinson, JP;
Schätzlein, AG;
Uchegbu, IF;
(2017)
T-shaped Peptide Amphiphiles Self Assemble into Nanofiber Networks.
Pharmaceutical Nanotechnology
, 5
(3)
pp. 215-219.
10.2174/2211738505666170828095937.
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Abstract
BACKGROUND: Conventional nanofiber forming peptide amphiphiles comprise a beta sheet forming, short peptide sequence with an alkyl chain attached at one terminus. We report the self-assembly of a peptide amphiphile possessing a mid-chain located alkyl substituent (a T-shaped peptide amphiphile) into nanofiber networks. METHOD: Peptide synthesis was carried out using standard 9-fluorenylmethoxycarbonyl solid phase peptide synthesis protocols, followed by covalent attachment of the alkyl chains to yield target peptide amphiphiles. Self-assembly was then studied using electron microscopy and coarse grained molecular dynamics simulations. RESULTS: T-shaped peptide amphiphiles self-assembled into nanofibers just like linear peptide amphiphiles, but then unlike linear peptide amphiphiles, T-shaped peptide amphiphiles formed inter-fiber associations and ultimately nanofiber networks. CONCLUSION: Changing the position of the alkyl chain in a peptide amphiphile from the terminal end of the peptide to the middle part of the peptide, to form a T-shaped peptide amphiphile, does not disrupt the molecular interactions required for the self-assembly of the peptide amphiphiles into nanofibers.
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