UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Characterization of a core region in the A2UCOE that confers effective anti-silencing activity

Zhang, F; Santilli, G; Thrasher, AJ; (2017) Characterization of a core region in the A2UCOE that confers effective anti-silencing activity. Scientific Reports , 7 , Article 10213. 10.1038/s41598-017-10222-3. Green open access

[thumbnail of Version of record]
Preview
Text (Version of record)
Zhang_Characterization_core_region.pdf - Published Version

Download (2MB) | Preview
[thumbnail of Supplementary data]
Preview
Text (Supplementary data)
Zhang_Characterization_core_region_Suppl.pdf

Download (648kB) | Preview

Abstract

We have previously shown that reliability of the A2UCOE in driving transgene expression can be attributed to its resistance to DNA methylation, and its ability to confer this property to linked regulatory sequences. In order to gain a better understanding of how resistance to DNA methylation from the A2UCOE is conferred, and whether the anti-silencing effect from the A2UCOE is confined within a core region, we evaluated the anti-silencing effect of different sub-domains. We found that maximal epigenetic regulatory activity was contained within a 455 bp element derived from the CBX3 region when tested in the context of a lentiviral vector in murine embryonic stem (ES) cells and human inducible pluripotent stem (iPS) cells. This region possessed an active chromatin signature, and operated effectively in cis to protect linked heterologous regulatory elements from methylation, thereby conferring stable transgene expression. Defined UCOE elements may be particularly useful for use in vectors where gene expression is desired in methylation-prone chromatin environments such as those encountered in pluripotent stem cells.

Type: Article
Title: Characterization of a core region in the A2UCOE that confers effective anti-silencing activity
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41598-017-10222-3
Publisher version: http://dx.doi.org/10.1038/s41598-017-10222-3
Language: English
Additional information: Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1573122
Downloads since deposit
148Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item