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Imp and Syp RNA-binding proteins govern decommissioning of Drosophila neural stem cells

Yang, C-P; Samuels, TJ; Huang, Y; Yang, L; Ish-Horowicz, D; Davis, I; Lee, T; (2017) Imp and Syp RNA-binding proteins govern decommissioning of Drosophila neural stem cells. Development , 144 (19) pp. 3454-3464. 10.1242/dev.149500. Green open access

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Abstract

The termination of the proliferation of Drosophila neural stem cells, also known as neuroblasts (NBs), requires a “decommissioning” phase that is controlled in a lineage-specific manner. Most NBs, with the exception of those of the Mushroom body (MB), are decommissioned by the ecdysone receptor and mediator complex causing them to shrink during metamorphosis, followed by nuclear accumulation of Prospero and cell cycle exit. Here, we demonstrate that the levels of Imp and Syp RNA-binding proteins regulate NB decommissioning. Descending Imp and ascending Syp expression have been shown to regulate neuronal temporal fate. We show that Imp levels decline slower in the MB than other central brain NBs. MB NBs continue to express Imp into pupation, and the presence of Imp prevents decommissioning partly by inhibiting the mediator complex. Late-larval induction of transgenic Imp prevents many non-MB NBs from decommissioning in early pupae. Moreover, the presence of abundant Syp in aged NBs permits Prospero accumulation that, in turn, promotes cell cycle exit. Together our results reveal that progeny temporal fate and progenitor decommissioning are co-regulated in protracted neuronal lineages.

Type: Article
Title: Imp and Syp RNA-binding proteins govern decommissioning of Drosophila neural stem cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.149500
Publisher version: http://doi.org/10.1242/dev.149500
Language: English
Additional information: Copyright © 2017. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Neural stem cell, RNA-binding protein, Mediator complex, Cell cycle exit, Mushroom body
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1572422
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