Yang, C-P; Samuels, TJ; Huang, Y; Yang, L; Ish-Horowicz, D; Davis, I; Lee, T; (2017) Imp and Syp RNA-binding proteins govern decommissioning of Drosophila neural stem cells. Development , 144 (19) pp. 3454-3464. 10.1242/dev.149500.

Preview

Text Ish-Horowicz_3454.full.pdf - Published Version Download (35MB) | Preview

Abstract

The termination of the proliferation of Drosophila neural stem cells, also known as neuroblasts (NBs), requires a “decommissioning” phase that is controlled in a lineage-specific manner. Most NBs, with the exception of those of the Mushroom body (MB), are decommissioned by the ecdysone receptor and mediator complex causing them to shrink during metamorphosis, followed by nuclear accumulation of Prospero and cell cycle exit. Here, we demonstrate that the levels of Imp and Syp RNA-binding proteins regulate NB decommissioning. Descending Imp and ascending Syp expression have been shown to regulate neuronal temporal fate. We show that Imp levels decline slower in the MB than other central brain NBs. MB NBs continue to express Imp into pupation, and the presence of Imp prevents decommissioning partly by inhibiting the mediator complex. Late-larval induction of transgenic Imp prevents many non-MB NBs from decommissioning in early pupae. Moreover, the presence of abundant Syp in aged NBs permits Prospero accumulation that, in turn, promotes cell cycle exit. Together our results reveal that progeny temporal fate and progenitor decommissioning are co-regulated in protracted neuronal lineages.

Download activity - last month

Export as CSVJSONXML

Download activity - last 12 months

Export as CSVJSONXML

Downloads by country - last 12 months

Export as CSVJSONXML

Archive Staff Only

View Item