Goosens, VJ;
Busch, A;
Georgiadou, M;
Castagnini, M;
Forest, KT;
Waksman, G;
Pelicic, V;
(2017)
Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili.
PNAS - Proceedings of the National Academy of Sciences of the United States of America
, 114
(25)
E4978-E4986.
10.1073/pnas.1618539114.
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Abstract
Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heterologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes are sufficient to promote filament assembly and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, which we have purified and characterized biochemically. Our results contribute to a better mechanistic understanding of the assembly of remarkable dynamic filaments nearly ubiquitous in prokaryotes.
| Type: | Article |
|---|---|
| Title: | Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1073/pnas.1618539114 |
| Publisher version: | http://doi.org/10.1073/pnas.1618539114 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, type IV pili, type IV filamentous nanomachines, filament assembly, synthetic biology, II SECRETION SYSTEM, INNER MEMBRANE PLATFORM, ASPARTIC-ACID PROTEASES, NEISSERIA-MENINGITIDIS, CRYSTAL-STRUCTURE, THERMUS-THERMOPHILUS, PREPILIN PEPTIDASES, TWITCHING MOTILITY, MYXOCOCCUS-XANTHUS, PROTEIN PILM |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1569937 |
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