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Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili

Goosens, VJ; Busch, A; Georgiadou, M; Castagnini, M; Forest, KT; Waksman, G; Pelicic, V; (2017) Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili. PNAS - Proceedings of the National Academy of Sciences of the United States of America , 114 (25) E4978-E4986. 10.1073/pnas.1618539114. Green open access

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Abstract

Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heterologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes are sufficient to promote filament assembly and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, which we have purified and characterized biochemically. Our results contribute to a better mechanistic understanding of the assembly of remarkable dynamic filaments nearly ubiquitous in prokaryotes.

Type: Article
Title: Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.1618539114
Publisher version: http://doi.org/10.1073/pnas.1618539114
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, type IV pili, type IV filamentous nanomachines, filament assembly, synthetic biology, II SECRETION SYSTEM, INNER MEMBRANE PLATFORM, ASPARTIC-ACID PROTEASES, NEISSERIA-MENINGITIDIS, CRYSTAL-STRUCTURE, THERMUS-THERMOPHILUS, PREPILIN PEPTIDASES, TWITCHING MOTILITY, MYXOCOCCUS-XANTHUS, PROTEIN PILM
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1569937
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