Ssufya, A; (2007) Proteomics analysis of cerebrospinal fluid in patients with idiopathic dormal pressure hydrocephalus. Doctoral thesis , UCL (University College London).

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Abstract

Idiopathic normal pressure hydrocephalus (INPH) is now recognized as a treatable cause of dementia. Recent estimates show that it accounts for 1-10% of patients with the diagnosis of dementia.. Although, surgical treatment by inserting a ventriculo-peritoneal shunt is the only known procedure to treat the symptoms, the selection of patients for surgery has always been problematic. Therefore, a pilot project was undertaken to see if by using the method of proteomics biomarkers for NPH could be identified for patients with this condition. Objectives The aim of the project was to use surface enhanced laser desorption ionisation time of flight mass spectrometry (SELDI-TOF MS) to look at the expression of protein in idiopathic NPH and control groups. If there are any changes in the expression of proteins between the two groups, the SELDI-TOF MS and sophisticated biomarker wizard software will detect these and identify the statistical relevance of the biomarker. Once a biomarker has been 'flagged', further studies will be used to obtain the identity of the protein. Methods and materials Sixteen CSF samples were analysed in this study. Five patients with trigeminal neuralgia and four patients with benign intracranial hypertension were used as controls seven patients with idiopathic normal pressure hydrocephalus comprised the study group. SELDI-TOF MS analysis of all 16 samples was performed using four different ProteinChip arrays. Nine (high molecular mass proteins) potential biomarkers of NPH have been detected by using high mass CM10 proteinChip with significant statistic results. No biomarkers were detected using the other arrays used in this study. Conclusions In this study, the SELDI-TOF MS technique has been used to study the proteins in the CSF of patients with INPH, and compare them with the proteome maps of the control groups. This has resulted in the detection of several significant changes in the CSF proteins of the patients with INPH when compared to the other two groups used as controls.

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