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Differential protein expression in the pilocarpine model of status epilepticus in rat hippocampus

Bamidele, A; (2005) Differential protein expression in the pilocarpine model of status epilepticus in rat hippocampus. Doctoral thesis , UCL (University College London). Green open access


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Epilepsy is the most common neurological condition. In those patients who require surgery for the treatment of their epilepsy, mesial temporal sclerosis (MTS) is the most common lesion identified. Up to 50% of these patients have a history of prolonged febrile convulsion in childhood, but the pathogenic mechanisms are still unknown. In this study a pilocarpine model of status epilepticus was used to study the events involved in hippocampal injury associated with status epilepticus. Magnetic resonance imaging (MRI) w as carried out prior to hippocampal extraction. To obtain a global view of the protein changes involved in MTS, a proteomic approach was used to characterise the protein changes in the hippocampus of the treated animals when compared with corresponding control animals. A protein map of the hippocampus ensured that all proteins which were expressed in only one group, those that were modified (post translational modifications), and those in which their quantities were altered could be identified. Using PDQuest software, quantitative analysis of the proteins separated by 2-dimensional electrophoresis (2DE) on polyacrylamide gels showed that eleven proteins species were up-regulated in the treated hippocampus compared with those from control animals. Qualitative analysis showed that two proteins spots were only expressed in the treated group. Mass spectrometric (MS) analysis of one of the spots identified it as heat shock protein beta_l (Hsp27). This result represents a new finding in the pilocarpine model of status epilepticus. This differential expression of HSP27 is in line with findings from the kainic acid model of epilepsy as well as resected temporal tissues of epilepsy patients. This study therefore provides data to support the view that the use of proteomics may be important in the understanding of the pathological processes following status epilepticus.

Type: Thesis (Doctoral)
Title: Differential protein expression in the pilocarpine model of status epilepticus in rat hippocampus
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Thesis digitised by ProQuest.
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
URI: https://discovery.ucl.ac.uk/id/eprint/1568257
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