Javed, A;
Christodoulou, J;
Cabrita, LD;
Orlova, EV;
(2017)
The ribosome and its role in protein folding: looking through a magnifying glass.
Acta crystallographica. Section D, Structural biology
, D73
pp. 509-521.
10.1107/S2059798317007446.
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Abstract
Protein folding, a process that underpins cellular activity, begins co-translationally on the ribosome. During translation, a newly synthesized polypeptide chain enters the ribosomal exit tunnel and actively interacts with the ribosome elements – the r-proteins and rRNA that line the tunnel – prior to emerging into the cellular milieu. While understanding of the structure and function of the ribosome has advanced significantly, little is known about the process of folding of the emerging nascent chain (NC). Advances in cryo-electron microscopy are enabling visualization of NCs within the exit tunnel, allowing early glimpses of the interplay between the NC and the ribosome. Once it has emerged from the exit tunnel into the cytosol, the NC (still attached to its parent ribosome) can acquire a range of conformations, which can be characterized by NMR spectroscopy. Using experimental restraints within molecular-dynamics simulations, the ensemble of NC structures can be described. In order to delineate the process of co-translational protein folding, a hybrid structural biology approach is foreseeable, potentially offering a complete atomic description of protein folding as it occurs on the ribosome.
| Type: | Article |
|---|---|
| Title: | The ribosome and its role in protein folding: looking through a magnifying glass |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1107/S2059798317007446 |
| Publisher version: | https://doi.org/10.1107/S2059798317007446 |
| Language: | English |
| Additional information: | This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Biochemistry & Molecular Biology, Biophysics, Crystallography, Ribosome, Nascent Chain, Protein Folding, NMR, Cryo-EM, Escherichia-Coli Ribosome, Nascent Polypeptide-Chains, Immune Electron-Microscopy, 5 S RNA, Angstrom Resolution, Messenger-Rna, Exit Tunnel, Structural Basis, 70S Ribosome, Mitochondrial Ribosome |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1566672 |
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