UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

QUESP and QUEST revisited – fast and accurate quantitative CEST experiments

Golay, XGGR; Zaiss, M; Angelovski, G; Demetriou, E; McMahon, MT; Scheffler, K; (2018) QUESP and QUEST revisited – fast and accurate quantitative CEST experiments. Magnetic Resonance in Medicine , 79 (3) pp. 1708-1721. 10.1002/mrm.26813. Green open access

[img]
Preview
Text (Article)
qCEST_QUESPQUEST_revisited_17_05.pdf - Accepted version
Available under License : See the attached licence file.

Download (453kB) | Preview
[img]
Preview
Text (Supplementary)
qCEST_QUESPQUEST_SUPP_03_24.pdf - Accepted version
Available under License : See the attached licence file.

Download (611kB) | Preview
[img]
Preview
Image (Image - Scheme 1)
FIG1_Scheme1_rev.tif - Accepted version
Available under License : See the attached licence file.

Download (85kB) | Preview
[img]
Preview
Image (Figure 2)
FIG2_MM_QUEST_QUESP_k_Zspec_hoch.pdf - Accepted version
Available under License : See the attached licence file.

Download (326kB) | Preview
[img]
Preview
Image (Figure 3)
FIG3_combined_leanfig.pdf - Accepted version
Available under License : See the attached licence file.

Download (72kB) | Preview
[img]
Preview
Image (Figure 4)
FIG4_QUEST_combined.pdf - Accepted version
Available under License : See the attached licence file.

Download (37kB) | Preview
[img]
Preview
Image (Figure 5)
FIG5_combined_QUESP_QUEST_1000_9000Hz_lean.pdf - Accepted version
Available under License : See the attached licence file.

Download (126kB) | Preview
[img]
Preview
Image (Figure 6)
FIG6_combined_GLY2_GLY4_lean.pdf - Accepted version
Available under License : See the attached licence file.

Download (335kB) | Preview
[img]
Preview
Image (Figure 7)
FIG7_Arrhenius.pdf - Accepted version
Available under License : See the attached licence file.

Download (55kB) | Preview
[img]
Preview
Image (Figure 8)
FIG8_expturbo_lean.pdf - Accepted version
Available under License : See the attached licence file.

Download (278kB) | Preview

Abstract

Purpose: Chemical exchange saturation transfer (CEST) NMR or MRI experiments allow detection of low concentrated molecules with enhanced sensitivity via their proton exchange with the abundant water pool. Be it endogenous metabolites or exogenous contrast agents, an exact quantification of the actual exchange rate is required to design optimal pulse sequences and/or specific sensitive agents. / Methods: Refined analytical expressions allow deeper insight and improvement of accuracy for common quantification techniques. The accuracy of standard quantification methodologies, such as quantification of exchange rate using varying saturation power or varying saturation time, is improved especially for the case of nonequilibrium initial conditions and weak labeling conditions, meaning the saturation amplitude is smaller than the exchange rate (γB1 < k). / Results: The improved analytical ‘quantification of exchange rate using varying saturation power/time’ (QUESP/QUEST) equations allow for more accurate exchange rate determination, and provide clear insights on the general principles to execute the experiments and to perform numerical evaluation. The proposed methodology was evaluated on the large-shift regime of paramagnetic chemical-exchange-saturation-transfer agents using simulated data and data of the paramagnetic Eu(III) complex of DOTA-tetraglycineamide. / Conclusions: The refined formulas yield improved exchange rate estimation. General convergence intervals of the methods that would apply for smaller shift agents are also discussed.

Type: Article
Title: QUESP and QUEST revisited – fast and accurate quantitative CEST experiments
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/mrm.26813
Publisher version: http://dx.doi.org/10.1002/mrm.26813
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Brain Repair and Rehabilitation
URI: https://discovery.ucl.ac.uk/id/eprint/1563548
Downloads since deposit
65Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item