Gordon, JE;
Costa, TRD;
Patel, RS;
Gonzalez-Rivera, C;
Sarkar, MK;
Orlova, EV;
Waksman, G;
(2017)
Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation.
Molecular Microbiology
, 105
(2)
pp. 273-293.
10.1111/mmi.13700.
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Abstract
Recent studies have shown that conjugation systems of Gram-negative bacteria are composed of distinct inner and outer membrane core complexes (IMCs and OMCCs, respectively). Here, we characterized the OMCC by focusing first on a cap domain that forms a channel across the outer membrane. Strikingly, the OMCC caps of the Escherichia coli pKM101 Tra and Agrobacterium tumefaciens VirB/VirD4 systems are completely dispensable for substrate transfer, but required for formation of conjugative pili. The pKM101 OMCC cap and extended pilus also are dispensable for activation of a Pseudomonas aeruginosa type VI secretion system (T6SS). Chimeric conjugation systems composed of the IMCpKM101 joined to OMCCs from the A. tumefaciens VirB/VirD4, E. coli R388 Trw, and Bordetella pertussis Ptl systems support conjugative DNA transfer in E. coli and trigger P. aeruginosa T6SS killing, but not pilus production. The A. tumefaciens VirB/VirD4 OMCC, solved by transmission electron microscopy, adopts a cage structure similar to the pKM101 OMCC. The findings establish that OMCCs are highly structurally and functionally conserved – but also intrinsically conformationally flexible – scaffolds for translocation channels. Furthermore, the OMCC cap and a pilus tip protein coregulate pilus extension but are not required for channel assembly or function.
| Type: | Article |
|---|---|
| Title: | Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/mmi.13700 |
| Publisher version: | http://dx.doi.org/10.1111/mmi.13700 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Microbiology, HELICOBACTER-PYLORI CAGL, CONJUGAL TRANSFER SYSTEM, BRUCELLA-SUIS VIRB8, INCN PLASMID PKM101, T-PILUS BIOGENESIS, AGROBACTERIUM-TUMEFACIENS, ESCHERICHIA-COLI, DNA TRANSFER, GENE-TRANSFER, CORE COMPLEX |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1557578 |
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