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Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms

Nienow, AW; Hewitt, CJ; Heathman, TRJ; Glyn, VAM; Fonte, GN; Hanga, MP; Coopman, K; (2016) Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms. Biochemical Engineering Journal , 108 pp. 24-29. 10.1016/j.bej.2015.08.003. Green open access

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Abstract

In our recent work in different bioreactors up to 2.5 L in scale, we have successfully cultured hMSCs using the minimum agitator speed required for complete microcarrier suspension, NJS. In addition, we also reported a scaleable protocol for the detachment from microcarriers in spinner flasks of hMSCs from two donors. The essence of the protocol is the use of a short period of intense agitation in the presence of enzymes such that the cells are detached; but once detachment is achieved, the cells are smaller than the Kolmogorov scale of turbulence and hence not damaged. Here, the same approach has been effective for culture at NJS and detachment in-situ in 15 mL ambr™ bioreactors, 100 mL spinner flasks and 250 mL Dasgip bioreactors. In these experiments, cells from four different donors were used along with two types of microcarrier with and without surface coatings (two types), four different enzymes and three different growth media (with and without serum), a total of 22 different combinations. In all cases after detachment, the cells were shown to retain their desired quality attributes and were able to proliferate. This agitation strategy with respect to culture and harvest therefore offers a sound basis for a wide range of scales of operation.

Type: Article
Title: Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bej.2015.08.003
Publisher version: https://doi.org/10.1016/j.bej.2015.08.003
Language: English
Additional information: Copyright © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Human mesenchymal stem cells; Bioreactors; Microcarriers; Mixing; Downstream processing; Harvest
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1557551
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