Ramakrishnan, L;
Uhlinger, K;
Dale, L;
Hamdoun, A;
Patel, S;
(2016)
ADP-Ribosyl Cyclases Regulate Early Development of the Sea Urchin.
Messenger
, 5
(1-2)
pp. 100-106.
10.1166/msr.2016.1052.
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Abstract
ADP-ribosyl cyclases are multifunctional enzymes involved in the metabolism of nucleotide derivatives necessary for Ca2+ signalling such as cADPR and NAADP. Although Ca2+ signalling is a critical regulator of early development, little is known of the role of ADP-ribosyl cyclases during embryogenesis. Here we analyze the expression, activity and function of ADP-ribosyl cyclases in the embryo of the sea urchin–a key organism for study of both Ca2+ signalling and embryonic development. ADP-ribosyl cyclase isoforms (SpARC1-4) showed unique changes in expression during early development. These changes were associated with an increase in the ratio of cADPR:NAADP production. Over-expression of SpARC4 (a preferential cyclase) disrupted gastrulation. Our data highlight the importance of ADP-ribosyl cyclases during embryogenesis.
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