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Pre/pro-B cells generate macrophage populations during homeostasis and inflammation

Audzevich, T; Bashford-Rogers, R; Mabbott, NA; Frampton, D; Freeman, TC; Potocnik, A; Kellam, P; (2017) Pre/pro-B cells generate macrophage populations during homeostasis and inflammation. Proceedings of The National Academy of Sciences of The United States of America (PNAS) , 114 (20) E3954-E3963. 10.1073/pnas.1616417114. Green open access

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Abstract

Most tissue-resident macrophages (Mφs) are believed to be derived prenatally and are assumed to maintain themselves throughout life by self-proliferation. However, in adult mice we identified a progenitor within bone marrow, early pro-B cell/fraction B, that differentiates into tissue Mφs. These Mφ precursors have non-rearranged B-cell receptor genes and coexpress myeloid (GR1, CD11b, and CD16/32) and lymphoid (B220 and CD19) lineage markers. During steady state, these precursors exit bone marrow, losing Gr1, and enter the systemic circulation, seeding the gastrointestinal system as well as pleural and peritoneal cavities but not the brain. While in these tissues, they acquire a transcriptome identical to embryonically derived tissue-resident Mφs. Similarly, these Mφ precursors also enter sites of inflammation, gaining CD115, F4/80, and CD16/32, and become indistinguishable from blood monocyte-derived Mφs. Thus, we have identified a population of cells within the bone marrow early pro-B cell compartment that possess functional plasticity to differentiate into either tissue-resident or inflammatory Mφs, depending on microenvironmental signals. We propose that these precursors represent an additional source of Mφ populations in adult mice during steady state and inflammation.

Type: Article
Title: Pre/pro-B cells generate macrophage populations during homeostasis and inflammation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.1616417114
Publisher version: http://doi.org/10.1073/pnas.1616417114
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, homeostasis, inflammation, macrophages, COLONY-STIMULATING FACTOR, PRO-B, LINEAGE COMMITMENT, BONE-MARROW, STEM-CELLS, IN-VITRO, MICE, PROGENITORS, EXPRESSION, FETAL
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/1555311
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