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Steady-state EB cap size fluctuations are determined by stochastic microtubule growth and maturation

Rickman, J; Duellberg, C; Cade, NI; Griffin, LD; Surrey, T; (2017) Steady-state EB cap size fluctuations are determined by stochastic microtubule growth and maturation. PNAS - Proceedings of the National Academy of Sciences , 114 (13) pp. 3427-3432. 10.1073/pnas.1620274114. Green open access

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Abstract

Growing microtubules are protected from depolymerization by the presence of a GTP or GDP/Pi cap. End-binding proteins of the EB1 family bind to the stabilizing cap, allowing monitoring of its size in real time. The cap size has been shown to correlate with instantaneous microtubule stability. Here we have quantitatively characterized the properties of cap size fluctuations during steady-state growth and have developed a theory predicting their timescale and amplitude from the kinetics of microtubule growth and cap maturation. In contrast to growth speed fluctuations, cap size fluctuations show a characteristic timescale, which is defined by the lifetime of the cap sites. Growth fluctuations affect the amplitude of cap size fluctuations; however, cap size does not affect growth speed, indicating that microtubules are far from instability during most of their time of growth. Our theory provides the basis for a quantitative understanding of microtubule stability fluctuations during steady-state growth.

Type: Article
Title: Steady-state EB cap size fluctuations are determined by stochastic microtubule growth and maturation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.1620274114
Publisher version: http://dx.doi.org/10.1073/pnas.1620274114
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, Microtubules, Dynamic Instability, GTP Cap, EB1, Biochemical Network, Ornstein-Uhlenbeck Process, Dynamic Instability, End Tracking, Individual Microtubules, Assembly Kinetics, GTP Hydrolysis, In-Vitro, Catastrophe, Microscopy, Noise
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of the Built Environment
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of the Built Environment > Bartlett School Env, Energy and Resources
URI: https://discovery.ucl.ac.uk/id/eprint/1552911
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