Diocou, S;
Volpe, A;
Jauregui-Osoro, M;
Boudjemeline, M;
Chuamsaamarkkee, K;
Man, F;
Blower, PJ;
... Fruhwirth, GO; + view all
(2017)
[(18)F]tetrafluoroborate-PET/CT enables sensitive tumor and metastasis in vivo imaging in a sodium iodide symporter-expressing tumor model.
Scientific Reports
, 7
, Article 946. 10.1038/s41598-017-01044-4.
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Abstract
Cancer cell metastasis is responsible for most cancer deaths. Non-invasive in vivo cancer cell tracking in spontaneously metastasizing tumor models still poses a challenge requiring highest sensitivity and excellent contrast. The goal of this study was to evaluate if the recently introduced PET radiotracer [(18)F]tetrafluoroborate ([(18)F]BF4(-)) is useful for sensitive and specific metastasis detection in an orthotopic xenograft breast cancer model expressing the human sodium iodide symporter (NIS) as a reporter. In vivo imaging was complemented by ex vivo fluorescence microscopy and γ-counting of harvested tissues. Radionuclide imaging with [(18)F]BF4(-) (PET/CT) was compared to the conventional tracer [(123)I]iodide (sequential SPECT/CT). We found that [(18)F]BF4(-) was superior due to better pharmacokinetics, i.e. faster tumor uptake and faster and more complete clearance from circulation. [(18)F]BF4(-)-PET was also highly specific as in all detected tissues cancer cell presence was confirmed microscopically. Undetected comparable tissues were similarly found to be free of metastasis. Metastasis detection by routine metabolic imaging with [(18)F]FDG-PET failed due to low standard uptake values and low contrast caused by adjacent metabolically active organs in this model. [(18)F]BF4(-)-PET combined with NIS expressing disease models is particularly useful whenever preclinical in vivo cell tracking is of interest.
| Type: | Article |
|---|---|
| Title: | [(18)F]tetrafluoroborate-PET/CT enables sensitive tumor and metastasis in vivo imaging in a sodium iodide symporter-expressing tumor model |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41598-017-01044-4 |
| Publisher version: | http://dx.doi.org/10.1038/s41598-017-01044-4 |
| Additional information: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1552845 |
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