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Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite

Obata, A; Iwanaga, N; Terada, A; Jell, G; Kasuga, T; (2017) Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite. Journal of Materials Science , 52 (15) pp. 8942-8956. 10.1007/s10853-017-1057-y. Green open access

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Abstract

Silicate ions released from bioactive glasses and ceramics have been reported to stimulate osteogenic cell functions. Here, we evaluated osteoblast-like cell reactions to silicate ions released from two different types of materials, 45S5 bioactive glass (BG) and siloxane-doped vaterite (SiV), to investigate the influence of the ionic structure of silicate ions on osteoblast-like cell properties. BG and SiV powders were prepared by using melt-quenching and carbonation methods, respectively. Aminopropyltriethoxysilane was used as a siloxane source of SiV. MC3T3-E1 and SaOS-2 cells were cultured in media containing dissolved BG or SiV ions (10–50 ppm of Si). Cell proliferation (metabolic activity), differentiation (alkaline phosphatase activity) and mineralisation (Ca deposition) were examined. 29Si NMR spectra demonstrated that Q0,1 species and T0–3 species were released from BG and SiV, respectively. Proliferation and mineralisation of the two types of cells were influenced by silicate ions released from BG and SiV in a concentration-dependent manner. In particular, there were significant differences (P < 0.05) in the degree of proliferation and Ca deposition levels in SaOS-2 cells treated with dissolved BG and SiV ions. Furthermore, Ca deposition in SaOS-2 cells was influenced by both the presence of silicate ions and the duration of exposure of cells to them. The structure of silicate ions influenced the proliferation and mineralisation of SaOS-2 cells incubated for different time periods in culture media containing different Si concentrations. Understanding the effect of Si on bone cell behaviour will enable a design-led approach to further BG optimisation.

Type: Article
Title: Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s10853-017-1057-y
Publisher version: http://dx.doi.org/10.1007/s10853-017-1057-y
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Technology, Materials Science, Multidisciplinary, Materials Science, IN-VITRO, SIGNALING PATHWAYS, BONE REGENERATION, GENE-EXPRESSION, DIFFERENTIATION, DISSOLUTION, PRODUCTS, SCAFFOLD, MINERALIZATION, PROLIFERATION
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/1552672
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