Voiculescu, Octavian;
Stern, Claudio D;
(2017)
Manipulating Gene Expression in the Chick Embryo.
In: Moulton, Hong M and Moulton, Jon D, (eds.)
Morpholino Oligomers: Methods and Protocols.
(pp. 105-114).
Humana Press (Springer): New York, NY, USA.
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Abstract
The chick embryo is a well-established representative of amniote embryos, which has been used to make many discoveries, including many major concepts, which have moved our knowledge of developmental biology in hugely important ways. The chick has a relatively compact genome and is easily amenable to embryological manipulations and in vivo imaging. Morpholino gene manipulations have been used in a variety of contexts, and constitute a quick and versatile molecular tool. Here we describe methods to deliver morpholinos to chick embryos, allowing targeting of specific cell populations at defined developmental stages, using two stages as examples: the epiblast of the embryo in the first day of incubation, when the primary germ layers of the embryo are specified, and in ovo electroporation of the neural tube as an example of a later stage. With slight modifications, these general methods can be used to target other embryonic tissues.
| Type: | Book chapter |
|---|---|
| Title: | Manipulating Gene Expression in the Chick Embryo |
| Location: | United States |
| ISBN-13: | 978-1-4939-6815-2 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-6817-6_9 |
| Publisher version: | http://dx.doi.org/10.1007/978-1-4939-6817-6_9 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Chick embryo; Electroporation; Morpholino; Epiblast; Neural plate; Ectoderm; Mesoderm; Endoderm |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1551513 |
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