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Super-resolution microscopy in the diagnosis of platelet granule disorders

Knight, AE; Gomez, K; Cutler, DF; (2017) Super-resolution microscopy in the diagnosis of platelet granule disorders. Expert Review of Hematology , 10 (5) pp. 375-381. 10.1080/17474086.2017.1315302. Green open access

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Abstract

INTRODUCTION: Platelet granule deficiencies lead to bleeding disorders, but their specific diagnosis typically requires whole mount transmission electron microscopy, which is often not available and has a number of important limitations. We recently proposed the use of advanced forms of fluorescence microscopy – the so-called ‘super-resolution’ microscopies – as a possible solution. AREAS COVERED: In this special report, we review the diagnosis of platelet granule deficiencies, and discuss how recent developments in fluorescence microscopy may be useful in improving diagnostic approaches to these and related disorders. In particular, we conclude that super-resolution fluorescence microscopy may have advantages over transmission electron microscopy in this application. EXPERT COMMENTARY: The value of the super-resolution microscopies has been amply demonstrated in research; however, their potential in diagnostic applications is ripe for further exploration. Hematology is a field particularly likely to benefit because of the relative simplicity of sample preparation. We anticipate that the costs of the necessary instrumentation will continue to fall rapidly, making these technologies widely accessible to clinicians.

Type: Article
Title: Super-resolution microscopy in the diagnosis of platelet granule disorders
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/17474086.2017.1315302
Publisher version: http://dx.doi.org/10.1080/17474086.2017.1315302
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Super-resolution microscopy, structured illumination microscopy, platelet disorders, dense granules, Hermansky-Pudlak syndrome
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
URI: https://discovery.ucl.ac.uk/id/eprint/1551345
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