UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Use of whole genome sequencing in the Dutch Acute HCV in HIV study: focus on transmitted antiviral resistance

Christiansen, MT; Hullegie, SJ; Schutten, M; Einer-Jensen, K; Tutill, HJ; Breuer, J; Rijnders, BJA; (2017) Use of whole genome sequencing in the Dutch Acute HCV in HIV study: focus on transmitted antiviral resistance. Clinical Microbiology and Infection , 23 (2) 123.e1-123.e4. 10.1016/j.cmi.2016.09.018. Green open access

[thumbnail of Breuer_use of whole genome_Dutch Acute HCV in HIV study_.pdf]
Preview
Text
Breuer_use of whole genome_Dutch Acute HCV in HIV study_.pdf - Accepted Version

Download (444kB) | Preview

Abstract

Objective Within HIV-positive men having sex with men, the epidemic of hepatitis C virus (HCV) is ongoing. Transmission of resistant variants of HCV after failure of treatment with directly acting antivirals (DAA) could be a major threat to the effectivity of therapy. We determined whether HCV-resistant variants to DAAs were prevalent amongst patients with an acute HCV infection diagnosed in 2013 and 2014 in the Netherlands. Methods Target enrichment for viral nucleic acid separation and deep sequencing were used to recover whole HCV genomes of 50 patients with an acute HCV infection. The genomes were assembled by de novo assembly and analysed for known DAA resistance mutations. Results In acute HCV infected treatment-naive patients, the relevant resistance-associated substitutions were Q80K (40%) in NS3/4a, M28V (24%) and Q30H combined with Y93H (2%) in NS5A and M414T (2%) or S556G (2%) in NS5b. Patients whose HCV infection failed to respond to boceprevir, peginterferon and ribavirin therapy developed mutations in NS3 at position T54A and R155K. Conclusions Target enrichment and whole genome sequencing were successfully applied directly on clinical samples from patients with an acute HCV infection.

Type: Article
Title: Use of whole genome sequencing in the Dutch Acute HCV in HIV study: focus on transmitted antiviral resistance
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.cmi.2016.09.018
Publisher version: http://doi.org/10.1016/j.cmi.2016.09.018
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Infectious Diseases, Microbiology, Acute hepatitis C, HIV, Protease inhibitors, Resistance, Treatment, HEPATITIS-C
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1550750
Downloads since deposit
115Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item