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Fluid Dynamic Characterization of a Laboratory Scale Rocked Bag Bioreactor

Marsh, DTJ; Odeleye, AOO; Ducci, A; Osborne, MD; Lye, GJ; Micheletti, M; (2017) Fluid Dynamic Characterization of a Laboratory Scale Rocked Bag Bioreactor. AIChE Journal , 63 (9) pp. 4177-4187. 10.1002/aic.15734. Green open access

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Abstract

Single-use technology is being widely adopted for the manufacture of biotherapeutics and cell therapy products. Rocked single-use bioreactors in particular have been commonly used, however, the hydrodynamics have rarely been characterized and are poorly understood. In this work, phase-resolved Particle Image Velocimetry and high frequency visual fluid tracking were used to investigate the flow pattern and velocity characteristics for the first time. The studies were performed on an optically accessible mimic of a Sartorius 2L CultiBag at different conditions. Wave formation was observed and higher rocking speeds caused the fluid to move proportionately out of phase with respect to the platform. Dimensional comparisons of fluid velocities with conventional bioreactors suggest that similar fluid dynamics characteristics can be achieved between rocked and stirred configurations. These results provide a first insight into the fluid dynamics of a novel bioreactor type at relevant process conditions supporting the generation of scale translation laws.

Type: Article
Title: Fluid Dynamic Characterization of a Laboratory Scale Rocked Bag Bioreactor
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/aic.15734
Publisher version: http://dx.doi.org/10.1002/aic.15734
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: mixing; single-use; bioreactor; wave; particle image velocimetry
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1547744
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