Roycroft, A;
(2017)
The role and regulation of cell matrix adhesions during contact inhibition of locomotion.
Doctoral thesis , UCL (University College London).
Preview |
Text
Roycroft_Thesis_final_AR.pdf Download (4MB) | Preview |
Abstract
Contact inhibition of locomotion (CIL) was first characterised over 60 years ago and defined as the behaviour of a cell to cease its continued migration in the same direction upon a collision with another cell. It has been implicated in multiple developmental processes including the precise dispersion of haemocytes and the directional migration of the cranial neural crest. In addition its absence has been linked to metastasis in cancer. Although many molecular mechanisms have recently been implicated in CIL, the role of cell-matrix adhesions (CMAs) during this process remains unknown. It has been hypothesised that cellular forces play an important role in CIL; however the role of traction forces generated via CMAs and intercellular tension during CIL is unclear. In this present study neural crest cells are used to elucidate the role and regulation of CMAs during CIL. The findings presented here demonstrate a rapid disassembly of CMAs near the cell-cell contact between colliding cells. This disassembly is shown to be dependent upon the formation of N-cadherin based cellcell adhesions and driven by Src and FAK kinase activity. Furthermore this rapid disassembly of CMAs during CIL leads to a redistribution of intercellular forces from the substrate to the cell-cell contact and is essential to drive cell-cell separation after a collision.
| Type: | Thesis (Doctoral) |
|---|---|
| Title: | The role and regulation of cell matrix adhesions during contact inhibition of locomotion |
| Event: | UCL (University College London) |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| UCL classification: | UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1546509 |
Loading...
Loading...Loading...| 1. |  United States | 9 |
| 2. |  United Kingdom | 5 |
| 3. |  Hong Kong | 1 |
| 4. |  France | 1 |
| 5. |  China | 1 |
| 6. |  Argentina | 1 |
| 7. |  India | 1 |
| 8. |  Moldova, Republic of | 1 |
| 9. |  Russian Federation | 1 |
Archive Staff Only
 |
View Item |
