He, J;
Cooper, HM;
Reyes, A;
Di Re, M;
Sembongi, H;
Litwin, TR;
Gao, J;
... Holt, IJ; + view all
(2012)
Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.
Nucleic Acids Research
, 40
(13)
pp. 6109-6121.
10.1093/nar/gks266.
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Abstract
Mitochondrial ribosomes and translation factors co-purify with mitochondrial nucleoids of human cells, based on affinity protein purification of tagged mitochondrial DNA binding proteins. Among the most frequently identified proteins were ATAD3 and prohibitin, which have been identified previously as nucleoid components, using a variety of methods. Both proteins are demonstrated to be required for mitochondrial protein synthesis in human cultured cells, and the major binding partner of ATAD3 is the mitochondrial ribosome. Altered ATAD3 expression also perturbs mtDNA maintenance and replication. These findings suggest an intimate association between nucleoids and the machinery of protein synthesis in mitochondria. ATAD3 and prohibitin are tightly associated with the mitochondrial membranes and so we propose that they support nucleic acid complexes at the inner membrane of the mitochondrion.
| Type: | Article |
|---|---|
| Title: | Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/nar/gks266 |
| Publisher version: | http://dx.doi.org/10.1093/nar/gks266 |
| Language: | English |
| Additional information: | © The Author(s) 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Adenosine Triphosphatases, Cell Cycle Proteins, Cell Line, Tumor, DNA, Mitochondrial, HEK293 Cells, Humans, Membrane Proteins, Mitochondria, Mitochondrial Proteins, Nuclear Proteins, Nucleoproteins, Protein Biosynthesis, RNA, RNA, Messenger, Repressor Proteins, Ribosomes |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1546414 |
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