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Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells

Tsuchiya, Y; Peak-Chew, S; Newell, C; Miller-Aidoo, S; Mangal, S; Zhyvoloup, A; Baković, J; ... Gout, I; + view all (2017) Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. Biochemical Journal , 474 (14) pp. 2489-2508. 10.1042/BCJ20170129. Green open access

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Abstract

Coenzyme A (CoA) is an obligatory cofactor in all branches of life. CoA and its derivatives are involved in major metabolic pathways, allosteric interactions and the regulation of gene expression. Abnormal biosynthesis and homeostasis of CoA and its derivatives have been associated with various human pathologies, including cancer, diabetes and neurodegeneration. Using an anti-CoA monoclonal antibody and mass spectrometry we identified a wide range of cellular proteins which are modified by covalent attachment of CoA to cysteine thiols (CoAlation). We show that protein CoAlation is a reversible post-translational modification that is induced in mammalian cells and tissues by oxidising agents and metabolic stress. A number of key cellular enzymes were found to be CoAlated in vitro and in vivo in ways that modified their activities. Our study reveals that protein CoAlation is a widespread post-translational modification which may play an important role in redox regulation under physiological and pathophysiological conditions.

Type: Article
Title: Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1042/BCJ20170129
Publisher version: http://dx.doi.org/10.1042/BCJ20170129
Language: English
Additional information: © 2017 The Author(s) This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND).
Keywords: Coenzyme A, metabolic and oxidative stress, post translational modification, proteomics
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1546387
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