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Appraisal of fluid flow in a shaken bioreactor with conical bottom at different operating conditions

Rodriguez, G; Pieralisi, I; Anderlei, T; Ducci, A; Micheletti, M; (2015) Appraisal of fluid flow in a shaken bioreactor with conical bottom at different operating conditions. Chemical Engineering Research and Design , 108 pp. 186-197. 10.1016/j.cherd.2015.12.012. Green open access

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Abstract

The flow dynamics in cylindrical shaken bioreactors of different conical bottom geometries is investigated by means of phase-resolved particle image velocimetry. Amid the variety of shaken vessels used for bioprocess applications, a cylindrical bioreactor with a conical bottom geometry was selected to assess its potential application in three-dimensional cultures, and improve solid suspension in shaken systems. This work builds upon the study of Weheliye et al. (2013. AIChE J. 59, 344) for a flat bottom with the objective to evaluate the effects of conical shaped bottoms of different heights on the fluid flow under different operating conditions with water being the working fluid. The results provide evidence that the presence of the conical bottom affects the transition from laminar to turbulent flow documented by Weheliye et al. (2013. AIChE J. 59, 344). The conical bottom with the greatest height investigated extended significantly the range of speeds over which flow transition occurs, with high intensity vortical structures spanning over the entire height of the bioreactor at lower speeds than those reported for a flat bottom geometry. This combined with the observed higher levels of kinetic energy should provide more efficient mechanisms for solid suspension.

Type: Article
Title: Appraisal of fluid flow in a shaken bioreactor with conical bottom at different operating conditions
Location: St Petersburg, RUSSIA
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.cherd.2015.12.012
Publisher version: http://doi.org/10.1016/j.cherd.2015.12.012
Language: English
Additional information: Copyright © 2016 Elsevier Ltd. All rights reserved. This is an Open Access article made available under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International license (CC BY-NC-ND 4.0). This license allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licenses are available at http://creativecommons.org/ licenses/by/4.0. Access may be initially restricted by the publisher.
Keywords: Shaken bioreactor; Conical bottom; Mixing dynamics; PIV
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1546227

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