McCoy, LE;
Burton, DR;
(2017)
Identification and specificity of broadly neutralizing antibodies against HIV.
Immunological Reviews
, 275
(1)
pp. 11-20.
10.1111/imr.12484.
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Abstract
Beginning in 2009, studies of the humoral responses of HIV-positive individuals have led to the identification of scores, if not hundreds, of antibodies that are both broadly reactive and potently neutralizing. This development has provided renewed impetus toward an HIV vaccine and led directly to the development of novel immunogens. Advances in identification of donors with the most potent and broad anti-HIV serum neutralizing responses were crucial in this effort. Equally, development of methods for the rapid generation of human antibodies from these donors was pivotal. Primarily these methods comprise single B-cell culture coupled to high-throughput neutralization screening and flow cytometry-based sorting of single B cells using HIV envelope protein baits. In this review, the advantages and disadvantages of these methodologies are discussed in the context of the specificities targeted by individual antibodies and the need for further improvements to evaluate HIV vaccine candidates.
| Type: | Article |
|---|---|
| Title: | Identification and specificity of broadly neutralizing antibodies against HIV |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/imr.12484 |
| Publisher version: | http://dx.doi.org/10.1111/imr.12484 |
| Language: | English |
| Additional information: | © 2017 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | B cells, HIV, monoclonal antibody isolation, neutralization |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1543369 |
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