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Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor

Carvalho, F; Marques, M; Fernandes, P; (2017) Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor. Catalysts , 7 (2) , Article 42. 10.3390/catal7020042. Green open access

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Abstract

Microscale technology has been increasingly used in chemical synthesis up to production scale, but in biocatalysis the implementation has been proceeding at a slower pace. In this work, the design of a low cost and versatile continuous flow enzyme microreactor is described that illustrates the potential of microfluidic reactors for both the development and characterization of biocatalytic processes. The core structure of the developed reactor consists of an array of capillaries with 450 μm of inner diameter with their inner surface functionalized with (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde where Saccharomyces cerevisiae invertase was covalently bound. The production of invert sugar syrup through enzymatic sucrose hydrolysis was used as model system. Once the microreactor assembly reproducibility and the immobilized enzyme behavior were established, the evaluation of the immobilized enzyme kinetic parameters was carried out at flow rates ranging from 20.8 to 219.0 μL·min−1 and substrate concentrations within 2.0%–10.0% (w/v). Despite the impact of immobilization on the kinetic parameters, viz. Km(app) was increased two fold and Kcat showed a 14-fold decrease when compared to solution phase invertase, the immobilization proved highly robust. For a mean residence time of 48.8 min, full conversion of 5.0% (w/v) sucrose was observed over 20 days.

Type: Article
Title: Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/catal7020042
Publisher version: http://dx.doi.org/10.3390/catal7020042
Language: English
Additional information: Copyright © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: microchannel reactor; biocatalysis; enzyme immobilization; sucrose hydrolysis; continuous processing
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1541003
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