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Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells

Baud, A; Wessely, F; Mazzacuva, F; McCormick, J; Camuzeaux, S; Heywood, WE; Little, D; ... Mills, K; + view all (2017) Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells. Analytical Chemistry , 89 (4) pp. 2440-2448. 10.1021/acs.analchem.6b04368. Green open access

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Abstract

Induced pluripotent stem cells have great potential as a human model system in regenerative medicine, disease modeling, and drug screening. However, their use in medical research is hampered by laborious reprogramming procedures that yield low numbers of induced pluripotent stem cells. For further applications in research, only the best, competent clones should be used. The standard assays for pluripotency are based on genomic approaches, which take up to 1 week to perform and incur significant cost. Therefore, there is a need for a rapid and cost-effective assay able to distinguish between pluripotent and nonpluripotent cells. Here, we describe a novel multiplexed, high-throughput, and sensitive peptide-based multiple reaction monitoring mass spectrometry assay, allowing for the identification and absolute quantitation of multiple core transcription factors and pluripotency markers. This assay provides simpler and high-throughput classification into either pluripotent or nonpluripotent cells in 7 min analysis while being more cost-effective than conventional genomic tests.

Type: Article
Title: Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.analchem.6b04368
Publisher version: http://dx.doi.org/10.1021/acs.analchem.6b04368
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1540468
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