Bradley, RS;
Robinson, IK;
Yusuf, M;
(2016)
3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds.
Macromolecular Bioscience
, 17
(2)
, Article 1600236. 10.1002/mabi.201600236.
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Abstract
Here, it is demonstrated that X-ray nanotomography with Zernike phase contrast can be used for 3D imaging of cells grown on electrospun polymer scaffolds. The scaffold fibers and cells are simultaneously imaged, enabling the influence of scaffold architecture on cell location and morphology to be studied. The high resolution enables subcellular details to be revealed. The X-ray imaging conditions were optimized to reduce scan times, making it feasible to scan multiple regions of interest in relatively large samples. An image processing procedure is presented which enables scaffold characteristics and cell location to be quantified. The procedure is demonstrated by comparing the ingrowth of cells after culture for 3 and 6 days.
| Type: | Article |
|---|---|
| Title: | 3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/mabi.201600236 |
| Publisher version: | http://dx.doi.org/10.1002/mabi.201600236 |
| Language: | English |
| Additional information: | This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Cell infiltration; electrospun scaffolds; imaging; microstructure; X-ray nanotomography |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1537569 |
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