UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Reporter gene imaging of targeted T cell immunotherapy in recurrent glioma

Keu, KV; Witney, TH; Yaghoubi, S; Rosenberg, J; Kurien, A; Magnusson, R; Williams, J; ... Gambhir, SS; + view all (2017) Reporter gene imaging of targeted T cell immunotherapy in recurrent glioma. Science Translational Medicine , 9 (373) , Article eaag2196. 10.1126/scitranslmed.aag2196. Green open access

[thumbnail of Keu_Reporter gene imaging of targeted T cell immunotherapy in recurrent glioma.pdf]
Preview
Text
Keu_Reporter gene imaging of targeted T cell immunotherapy in recurrent glioma.pdf - Accepted Version

Download (1MB) | Preview

Abstract

High-grade gliomas are aggressive cancers that often become rapidly fatal. Immunotherapy using CD8(+) cytotoxic T lymphocytes (CTLs), engineered to express both herpes simplex virus type 1 thymidine kinase (HSV1-TK) and interleukin-13 (IL-13) zetakine chimeric antigen receptor (CAR), is a treatment strategy with considerable potential. To optimize this and related immunotherapies, it would be helpful to monitor CTL viability and trafficking to glioma cells. We show that noninvasive positron emission tomography (PET) imaging with 9-[4-[(18)F]fluoro-3-(hydroxymethyl)butyl]guanine ([(18)F]FHBG) can track HSV1-tk reporter gene expression present in CAR-engineered CTLs. [(18)F]FHBG imaging was safe and enabled the longitudinal imaging of T cells stably transfected with a PET reporter gene in patients. Further optimization of this imaging approach for monitoring in vivo cell trafficking should greatly benefit various cell-based therapies for cancer.

Type: Article
Title: Reporter gene imaging of targeted T cell immunotherapy in recurrent glioma
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1126/scitranslmed.aag2196
Publisher version: https://doi.org/10.1126/scitranslmed.aag2196
Language: English
Additional information: Copyright © 2017 American Association for the Advancement of Science. This is the author's version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science Translational Medicine 9(373), 18 January 2017, doi: 10.1126/scitranslmed.aag2196.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/1537090
Downloads since deposit
236Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item