Jolly, JK;
Wagner, SK;
Moules, J;
Gekeler, F;
Webster, AR;
Downes, SM;
MacLaren, RE;
(2016)
A Novel Method for Quantitative Serial Autofluorescence Analysis in Retinitis Pigmentosa Using Image Characteristics.
Translational Vision Science & Technology
, 5
(6)
, Article 10. 10.1167/tvst.5.6.10.
Preview |
Text
Jolly_Novel_Method_for_Quantitative_Serial.pdf - Published Version Download (587kB) | Preview |
Abstract
PURPOSE: Identifying potential biomarkers for disease progression in retinitis pigmentosa (RP) is highly relevant now that gene therapy and other treatments are in clinical trial. Here we report a novel technique for analysis of short-wavelength autofluorescence (AF) imaging to quantify defined regions of AF in RP patients. METHODS: Fifty-five–degree AF images were acquired from 12 participants with RP over a 12-month period. Of these, five were identified as having a hyperfluorescent annulus. A standard Cartesian coordinate system was superimposed on images with the fovea as the origin and eight bisecting lines traversing the center at 45 degrees to each other. Spatial extraction software was programmed to highlight pixels corresponding to varying degrees of percentile fluorescence such that the parafoveal AF ring was mapped. Distance between the fovea and midpoint of the AF ring was measured. Percentage of low luminance areas was utilized as a measure of atrophy. RESULTS: The hyperfluorescent ring was most accurately mapped using the 70th percentile of fluorescence. Both the AF ring and peripheral hypofluorescence showed robust repeatability at all time points noted (P = 0.93). CONCLUSIONS: Both a hypofluorescent ring and retinal pigment epithelium atrophy were present on a significant proportion of RP patients and were consistently mapped over a 12-month period. There is potential extrapolation of this methodology to wide-field imaging as well as other retinal dystrophies. This anatomical change may provide a useful anatomical biomarker for assessing treatment end points in RP. TRANSLATIONAL RELEVANCE: Spatial extraction software can be a valuable tool in the assessment of ophthalmic imaging data.
| Type: | Article |
|---|---|
| Title: | A Novel Method for Quantitative Serial Autofluorescence Analysis in Retinitis Pigmentosa Using Image Characteristics |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1167/tvst.5.6.10 |
| Publisher version: | http://dx.doi.org/10.1167/tvst.5.6.10 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0). |
| Keywords: | retinitis pigmentosa; autofluorescence; quantification; retinal degeneration |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1536617 |
Archive Staff Only
 |
View Item |
