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Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments

Mayer, MJ; Juodeikis, R; Brown, IR; Frank, S; Palmer, DJ; Deery, E; Beal, DM; ... Warren, MJ; + view all (2016) Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments. Scientific Reports , 6 , Article 36899. 10.1038/srep36899. Green open access

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Abstract

Bacterial microcompartments (BMCs) are proteinaceous organelles that are found in a broad range of bacteria and are composed of an outer shell that encases an enzyme cargo representing a specific metabolic process. The outer shell is made from a number of different proteins that form hexameric and pentameric tiles, which interact to allow the formation of a polyhedral edifice. We have previously shown that the Citrobacter freundii BMC associated with 1,2-propanediol utilization can be transferred into Escherichia coli to generate a recombinant BMC and that empty BMCs can be formed from just the shell proteins alone. Herein, a detailed structural and proteomic characterization of the wild type BMC is compared to the recombinant BMC and a number of empty BMC variants by 2D-gel electrophoresis, mass spectrometry, transmission electron microscopy (TEM) and atomic force microscopy (AFM). Specifically, it is shown that the wild type BMC and the recombinant BMC are similar in terms of composition, size, shape and mechanical properties, whereas the empty BMC variants are shown to be smaller, hollow and less malleable.

Type: Article
Title: Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/srep36899
Publisher version: http://doi.org/10.1038/srep36899
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, B-12-DEPENDENT 1,2-PROPANEDIOL DEGRADATION, PENTAMERIC VERTEX PROTEINS, SEROVAR TYPHIMURIUM LT2, SALMONELLA-ENTERICA, CARBOXYSOME SHELL, INCLUSIONS CARBOXYSOMES, ELECTRON CRYOTOMOGRAPHY, ESCHERICHIA-COLI, ORGANELLES, NEAPOLITANUS
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1535669
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