UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature

van der Putten, MA; MacKenzie, LE; Davies, AL; Fernandez-Ramos, J; Desai, RA; Smith, KJ; Harvey, AR; (2017) A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature. Physiological Measurement , 38 (2) pp. 205-218. 10.1088/1361-6579/aa5527. Green open access

[img]
Preview
Text
Davies_a multispectral microscope_in vivo_.pdf - Accepted version

Download (4MB) | Preview

Abstract

Quantification of blood oxygen saturation (SO2) in vivo is essential for understanding the pathogenesis of diseases in which hypoxia is thought to play a role, including inflammatory disorders such as multiple sclerosis (MS) and rheumatoid arthritis (RA). We describe a low-cost multispectral microscope and oximetry technique for calibration-free absolute oximetry of surgically exposed blood vessels in vivo. We imaged the vasculature of the dorsal spinal cord in healthy rats, and varied inspired oxygen (FiO2) in order to evaluate the sensitivity of the imaging system to changes in SO2. The venous SO2 was calculated as 67.8  ±  10.4% (average  ±  standard deviation), increasing to 83.1  ±  11.6% under hyperoxic conditions (100% FiO2) and returning to 67.4  ±  10.9% for a second normoxic period; the venous SO2 was 50.9  ±  15.5% and 29.2  ±  24.6% during subsequent hypoxic states (18% and 15% FiO2 respectively). We discuss the design and performance of our multispectral imaging system, and the future scope for extending this oximetry technique to quantification of hypoxia in inflamed tissue.

Type: Article
Title: A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature
Open access status: An open access version is available from UCL Discovery
DOI: 10.1088/1361-6579/aa5527
Publisher version: https://doi.org/10.1088/1361-6579/aa5527
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Technology, Biophysics, Engineering, Biomedical, Physiology, Engineering, multispectral imaging, oximetry, spinal cord vasculature, OPTICAL COHERENCE TOMOGRAPHY, RETINAL VESSEL OXIMETRY, OXYGEN-SATURATION, LIGHT PATHS, HYPOXIA, MICROVASCULATURE, HEMOGLOBIN, DISEASE, BLOOD, MODEL
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neuroinflammation
URI: https://discovery.ucl.ac.uk/id/eprint/1534684
Downloads since deposit
111Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item