van der Putten, MA;
MacKenzie, LE;
Davies, AL;
Fernandez-Ramos, J;
Desai, RA;
Smith, KJ;
Harvey, AR;
(2017)
A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature.
Physiological Measurement
, 38
(2)
pp. 205-218.
10.1088/1361-6579/aa5527.
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Abstract
Quantification of blood oxygen saturation (SO2) in vivo is essential for understanding the pathogenesis of diseases in which hypoxia is thought to play a role, including inflammatory disorders such as multiple sclerosis (MS) and rheumatoid arthritis (RA). We describe a low-cost multispectral microscope and oximetry technique for calibration-free absolute oximetry of surgically exposed blood vessels in vivo. We imaged the vasculature of the dorsal spinal cord in healthy rats, and varied inspired oxygen (FiO2) in order to evaluate the sensitivity of the imaging system to changes in SO2. The venous SO2 was calculated as 67.8 ± 10.4% (average ± standard deviation), increasing to 83.1 ± 11.6% under hyperoxic conditions (100% FiO2) and returning to 67.4 ± 10.9% for a second normoxic period; the venous SO2 was 50.9 ± 15.5% and 29.2 ± 24.6% during subsequent hypoxic states (18% and 15% FiO2 respectively). We discuss the design and performance of our multispectral imaging system, and the future scope for extending this oximetry technique to quantification of hypoxia in inflamed tissue.
| Type: | Article |
|---|---|
| Title: | A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1088/1361-6579/aa5527 |
| Publisher version: | https://doi.org/10.1088/1361-6579/aa5527 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Technology, Biophysics, Engineering, Biomedical, Physiology, Engineering, multispectral imaging, oximetry, spinal cord vasculature, OPTICAL COHERENCE TOMOGRAPHY, RETINAL VESSEL OXIMETRY, OXYGEN-SATURATION, LIGHT PATHS, HYPOXIA, MICROVASCULATURE, HEMOGLOBIN, DISEASE, BLOOD, MODEL |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neuroinflammation |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1534684 |
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