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Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey

Gardiner, C; Vizio, DD; Sahoo, S; Théry, C; Witwer, KW; Wauben, M; Hill, AF; (2016) Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. Journal of Extracellular Vesicles , 5 , Article 32945. 10.3402/jev.v5.32945. Green open access

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Abstract

Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed.

Type: Article
Title: Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey
Open access status: An open access version is available from UCL Discovery
DOI: 10.3402/jev.v5.32945
Publisher version: http://dx.doi.org/10.3402/jev.v5.32945
Language: English
Additional information: © 2016 Chris Gardiner et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Extracellular vesicles; isolation; characterization; purification; flow cytometry; proteomics; RNA analysis; single vesicle analysis
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: https://discovery.ucl.ac.uk/id/eprint/1534557
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