UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

The Local and Systemic Immune Response to Intrauterine LPS in the Prepartum Mouse

Edey, LF; O'Dea, KP; Herbert, BR; Hua, R; Waddington, SN; MacIntyre, DA; Bennett, PR; ... Johnson, MR; + view all (2016) The Local and Systemic Immune Response to Intrauterine LPS in the Prepartum Mouse. Biology of Reproduction , 95 (6) , Article 125. 10.1095/biolreprod.116.143289. Green open access

[thumbnail of Published article]
Preview
Text (Published article)
Edey_Local_Systemic_Immune.pdf - Published Version

Download (1MB) | Preview
[thumbnail of Supplementary data]
Preview
Text (Supplementary data)
Edey_Local_Systemic_Immune_Suppl.pdf

Download (743kB) | Preview

Abstract

Inflammation plays a key role in human term and preterm labor (PTL). Intrauterine LPS has been widely used to model inflammation-induced complications of pregnancy, including PTL. It has been shown to induce an intense myometrial inflammatory cell infiltration, but the role of LPS-induced inflammatory cell activation in labor onset and fetal demise is unclear. We investigated this using a mouse model of PTL, where an intrauterine injection of 10 μg of LPS (serotype 0111:B4) was given at E16 of CD1 mouse pregnancy. This dose induced PTL at an average of 12.7 h postinjection in association with 85% fetal demise. Flow cytometry showed that LPS induced a dramatic systemic inflammatory response provoking a rapid and marked leucocyte infiltration into the maternal lung and liver in association with increased cytokine levels. Although there was acute placental inflammatory gene expression, there was no corresponding increase in fetal brain inflammatory gene expression until after fetal demise. There was marked myometrial activation of NFκB and MAPK/AP-1 systems in association with increased chemokine and cytokine levels, both of which peaked with the onset of parturition. Myometrial macrophage and neutrophil numbers were greater in the LPS-injected mice with labor onset only; prior to labor, myometrial neutrophils and monocytes numbers were greater in PBS-injected mice, but this was not associated with an earlier onset of labor. These data suggest that intrauterine LPS induces parturition directly, independent of myometrial inflammatory cell infiltration, and that fetal demise occurs without fetal inflammation. Intrauterine LPS provokes a marked local and systemic inflammatory response but with limited inflammatory cell infiltration into the myometrium or placenta.

Type: Article
Title: The Local and Systemic Immune Response to Intrauterine LPS in the Prepartum Mouse
Open access status: An open access version is available from UCL Discovery
DOI: 10.1095/biolreprod.116.143289
Publisher version: https://doi.org/10.1095/biolreprod.116.143289
Language: English
Additional information: Copyright © 2016 by the Society for the Study of Reproduction, Inc. This article is available under a Creative Commons License 4.0 (Attribution-Non-Commercial), as described at http://creativecommons.org/licenses/by-nc/4.0.
Keywords: Keywords: chemokines, inflammation, leucocytes, lungs, myometrium
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Maternal and Fetal Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/1534417
Downloads since deposit
120Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item