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Neuronal retrograde transport of Borna disease virus occurs in signalling endosomes.

Charlier, CM; Debaisieux, S; Foret, C; Thouard, A; Schiavo, G; Gonzalez-Dunia, D; Malnou, CE; (2016) Neuronal retrograde transport of Borna disease virus occurs in signalling endosomes. J Gen Virol , 97 (12) pp. 3215-3224. 10.1099/jgv.0.000652. Green open access

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Abstract

Long-range axonal retrograde transport is a key mechanism for the cellular dissemination of neuroinvasive viruses, such as Borna disease virus (BDV), for which entry and egress sites are usually distant from the nucleus, where viral replication takes place. Although BDV is known to disseminate very efficiently in neurons, both in vivo and in primary cultures, the modalities of its axonal transport are still poorly characterized. In this work, we combined different methodological approaches, such as confocal microscopy and biochemical purification of endosomes, to study BDV retrograde transport. We demonstrate that BDV ribonucleoparticles (composed of the viral genomic RNA, nucleoprotein and phosphoprotein), as well as the matrix protein, are transported towards the nucleus into endocytic carriers. These specialized organelles, called signalling endosomes, are notably used for the retrograde transport of neurotrophins and activated growth factor receptors. Signalling endosomes have a neutral luminal pH and thereby offer protection against degradation during long-range transport. This particularity could allow the viral particles to be delivered intact to the cell body of neurons, avoiding their premature release in the cytoplasm.

Type: Article
Title: Neuronal retrograde transport of Borna disease virus occurs in signalling endosomes.
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1099/jgv.0.000652
Publisher version: http://dx.doi.org/10.1099/jgv.0.000652
Language: English
Additional information: © 2016 The Authors. Published by the Microbiology Society
Keywords: Neuron, axonal transport, Bornavirus, signalling endosomes
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/1533168
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