UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction

De Almeida, I; Oliveira, NMM; Randall, RA; Hill, CS; McCoy, JM; Stern, CD; (2017) Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction. Developmental Biology , 421 (2) pp. 161-170. 10.1016/j.ydbio.2016.12.001. Green open access

[img]
Preview
Text
Almeida_1-s2.0-S0012160616306960-main.pdf - Published version

Download (1MB) | Preview

Abstract

Hensen's node is the “organizer” of the avian and mammalian early embryo. It has many functions, including neural induction and patterning of the ectoderm and mesoderm. Some of the signals responsible for these activities are known but these do not explain the full complexity of organizer activity. Here we undertake a functional screen to discover new secreted factors expressed by the node at this time of development. Using a Signal Sequence Trap in yeast, we identify several candidates. Here we focus on Calreticulin. We show that in addition to its known functions in intracellular Calcium regulation and protein folding, Calreticulin is secreted, it can bind to BMP4 and act as a BMP antagonist in vivo and in vitro. Calreticulin is not sufficient to account for all organizer functions but may contribute to the complexity of its activity.

Type: Article
Title: Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ydbio.2016.12.001
Publisher version: http://doi.org/10.1016/j.ydbio.2016.12.001
Language: English
Additional information: © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Keywords: Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Neural plate, Gastrulation, Neurulation, Chick embryo, Phospho-Smad, Calnexin, Calcium-binding proteins, CALCIUM-BINDING PROTEIN, ENDOPLASMIC-RETICULUM, SARCOPLASMIC-RETICULUM, CARDIAC DEVELOPMENT, SIGNAL PEPTIDES, GENE-EXPRESSION, FACTORS CHORDIN, QUALITY-CONTROL, CHICK-EMBRYO, XENOPUS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1532459
Downloads since deposit
89Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item