Jennings, A;
Tyurikova, O;
Bard, L;
Zheng, K;
Semyanov, A;
Henneberger, C;
Rusakov, DA;
(2016)
Dopamine elevates and lowers astroglial Ca(2+) through distinct pathways depending on local synaptic circuitry.
Glia
, 65
(3)
pp. 447-459.
10.1002/glia.23103.
Preview |
Text
Jennings_et_al-2017-Glia.pdf Download (970kB) | Preview |
Abstract
Whilst astrocytes in culture invariably respond to dopamine with cytosolic Ca(2+) rises, the dopamine sensitivity of astroglia in situ and its physiological roles remain unknown. To minimize effects of experimental manipulations on astroglial physiology, here we monitored Ca(2+) in cells connected via gap junctions to astrocytes loaded whole-cell with cytosolic indicators in area CA1 of acute hippocampal slices. Aiming at high sensitivity of [Ca(2+) ] measurements, we also employed life-time imaging of the Ca(2+) indicator Oregon Green BAPTA-1. We found that dopamine triggered a dose-dependent, bidirectional Ca(2+) response in stratum radiatum astroglia, a jagged elevation accompanied and followed by below-baseline decreases. The elevation depended on D1/D2 receptors and engaged intracellular Ca(2+) storage and removal whereas the dopamine-induced [Ca(2+) ] decrease involved D2 receptors only and was sensitive to Ca(2+) channel blockade. In contrast, the stratum lacunosum moleculare astroglia generated higher-threshold dopamine-induced Ca(2+) responses which did not depend on dopamine receptors and were uncoupled from the prominent inhibitory action of dopamine on local perforant path synapses. Our findings thus suggest that a single neurotransmitter-dopamine-could either elevate or decrease astrocyte [Ca(2+) ] depending on the receptors involved, that such actions are specific to the regional neural circuitry and that they may be causally uncoupled from dopamine actions on local synapses. The results also indicate that [Ca(2+) ] elevations commonly detected in astroglia can represent the variety of distinct mechanisms acting on the microscopic scale. GLIA 2016.
| Type: | Article |
|---|---|
| Title: | Dopamine elevates and lowers astroglial Ca(2+) through distinct pathways depending on local synaptic circuitry |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/glia.23103 |
| Publisher version: | http://dx.doi.org/10.1002/glia.23103 |
| Language: | English |
| Additional information: | This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2016 The Authors Glia Published by Wiley Periodicals, Inc |
| Keywords: | Astroglia, calcium, dopamine, hippocampus, synaptic transmission |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1531050 |
Archive Staff Only
 |
View Item |
