UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

An anti-TNF-α antibody mimetic to treat ocular inflammation

Khalili, H; Lee, RW; Khaw, PT; Brocchini, S; Dick, AD; Copland, DA; (2016) An anti-TNF-α antibody mimetic to treat ocular inflammation. Scientific Reports , 6 , Article 36905. 10.1038/srep36905. Green open access

[thumbnail of Published article] Text (Published article)
Khalili_An anti-TNF antibody mimetic to treat ocular inflammation VoR.pdf

Download (1MB)
[thumbnail of Supplementary Figures]
Preview
Text (Supplementary Figures)
Khalili_An anti-TNF antibody mimetic to treat ocular inflammation Supplementary file.pdf

Download (658kB) | Preview

Abstract

Infliximab is an antibody that neutralizes TNF-α and is used principally by systemic administration to treat many inflammatory disorders. We prepared the antibody mimetic Fab-PEG-Fab (FpFinfliximab) for direct intravitreal injection to assess whether such formulations have biological activity and potential utility for ocular use. FpFinfliximab was designed to address side effects caused by antibody degradation and the presence of the Fc region. Surface plasmon resonance analysis indicated that infliximab and FpFinfliximab maintained binding affinity for both human and murine recombinant TNF-α. No Fc mediated RPE cellular uptake was observed for FpFinfliximab. Both Infliximab and FpFinfliximab suppressed ocular inflammation by reducing the number of CD45+ infiltrate cells in the EAU mice after a single intravitreal injection at the onset of peak disease. These results offer an opportunity to develop and formulate for ocular use, FpF molecules designed for single and potentially multiple targets using bi-specific FpFs.

Type: Article
Title: An anti-TNF-α antibody mimetic to treat ocular inflammation
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/srep36905
Publisher version: http://dx.doi.org/10.1038/srep36905
Language: English
Additional information: Copyright © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/1530804
Downloads since deposit
115Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item