Nixon-Abell, J;
Obara, CJ;
Weigel, AV;
Li, D;
Legant, WR;
Xu, CS;
Pasolli, HA;
... Lippincott-Schwartz, J; + view all
(2016)
Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER.
Science
, 354
(6311)
, Article 433. 10.1126/science.aaf3928.
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Abstract
The endoplasmic reticulum (ER) is an expansive, membrane-enclosed organelle that plays crucial roles in numerous cellular functions. We used emerging superresolution imaging technologies to clarify the morphology and dynamics of the peripheral ER, which contacts and modulates most other intracellular organelles. Peripheral components of the ER have classically been described as comprising both tubules and flat sheets. We show that this system consists almost exclusively of tubules at varying densities, including structures that we term ER matrices. Conventional optical imaging technologies had led to misidentification of these structures as sheets because of the dense clustering of tubular junctions and a previously uncharacterized rapid form of ER motion. The existence of ER matrices explains previous confounding evidence that had indicated the occurrence of ER "sheet" proliferation after overexpression of tubular junction-forming proteins.
| Type: | Article |
|---|---|
| Title: | Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1126/science.aaf3928 |
| Publisher version: | http://dx.doi.org/10.1126/science.aaf3928 |
| Language: | English |
| Additional information: | This is the author's version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science 354(6311), doi: 10.1126/science.aaf3928. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1528687 |
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