Wang, SX;
Acha, D;
Shah, AJ;
Hills, F;
Roitt, I;
Demosthenous, A;
Bayford, RH;
(2017)
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor.
Biosensors and Bioelectronics
, 92
pp. 482-488.
10.1016/j.bios.2016.10.077.
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Abstract
This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein – one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody–antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting.
| Type: | Article |
|---|---|
| Title: | Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bios.2016.10.077 |
| Publisher version: | http://dx.doi.org/10.1016/j.bios.2016.10.077 |
| Language: | English |
| Additional information: | Copyright © 2016. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher. |
| Keywords: | Alzheimer's disease; Biomarker; Electrochemical biosensor; Impedance; Tau |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1527523 |
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