UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

New Generation of Tunable Bioactive Shape Memory Mats Integrated with Genetically Engineered Proteins

Wu, X; Mahalingam, S; VanOosten, SK; Wisdom, C; Tamerler, C; Edirisinghe, M; (2017) New Generation of Tunable Bioactive Shape Memory Mats Integrated with Genetically Engineered Proteins. Macromolecular Bioscience , 17 (2) , Article 1600270. 10.1002/mabi.201600270. Green open access

[thumbnail of Published article]
Preview
Text (Published article)
Wu_New_Generation_Tunable.pdf - Published version

Download (2MB) | Preview
[thumbnail of Supplementary file 1] Video (Supplementary file 1)
Wu_New_Generation_Tunable_S1.mp4

Download (4MB)
[thumbnail of Supplementary file 2] Video (Supplementary file 2)
Wu_New_Generation_Tunable_S2.mp4

Download (6MB)

Abstract

Aligned poly(l-lactide)/poly(methyl methacrylate) binary blend fibers and mats loaded with a chimeric green fluorescence protein having a bioactive peptide with hydroxyapatite binding and mineralization property are prepared by pressurized gyration. The effect of processing parameters on the product morphologies, and the shape memory properties of these samples are investigated. Integration of hydroxyapatite nanoparticles into the fiber assembly is self-directed using the hydroxyapatite-binding property of the peptide genetically engineered to green fluorescence protein. Fluorescence microscopy analysis corroborated with Fourier transform infrared spectroscopy (FTIR) data confirms the integration of the chimeric protein with the fibers. An enzyme based remineralization assay is conducted to study the effects of peptide-mediated mineralization within the fiber mats. Raman and FTIR spectral changes observed following the peptide-mediated mineralization provides an initial step toward a soft-hard material transition. These results show that programmable shape memory properties can be obtained by incorporating genetically engineered bioactive peptide domains into polymer fibers.

Type: Article
Title: New Generation of Tunable Bioactive Shape Memory Mats Integrated with Genetically Engineered Proteins
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/mabi.201600270
Publisher version: http://dx.doi.org/10.1002/mabi.201600270
Language: English
Additional information: Copyright © 2016 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: genetically engineered proteins, peptide based mineralization, polymer, pressurized gyration, shape memory
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1524776
Downloads since deposit
57Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item