Jeffries, JWE;
Dawson, N;
Orengo, C;
Moody, TS;
Quinn, DJ;
Hailes, HC;
Ward, JM;
(2016)
Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis.
ChemistrySelect
, 1
(10)
pp. 2217-2220.
10.1002/slct.201600515.
Preview |
Text
ward_Metagenomics paper for RPS.pdf Download (1MB) | Preview |
Abstract
Biocatalytic reactions are increasingly being used as a sustainable strategy in organic synthesis and it is recognised that there is need for new enzyme discovery. To establish the utility and versatility of a metagenomics approach, metagenomic DNA extracted from the oral cavity was sequenced and used to create an in silico contig library. This enables individual open reading frames, operons or all the enzymes of a particular family to be identified and then retrieved from the original DNA by PCR. As proof of principle a lactate dehydrogenase, a malate dehydrogenase and transketolases were identified in silico, successfully cloned and assayed. This new enzyme retrieval sequence directed method gives constructive access to metagenomic diversity and importantly improves on the low hit rate experienced when using conventional metagenomic screens.
| Type: | Article |
|---|---|
| Title: | Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/slct.201600515 |
| Publisher version: | http://doi.org/10.1002/slct.201600515 |
| Language: | English |
| Additional information: | © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Jeffries, JWE; Dawson, N; Orengo, C; Moody, TS; Quinn, DJ; Hailes, HC; Ward, JM; (2016) Metagenome Mining: A Sequence Directed Strategy for the Retrieval of Enzymes for Biocatalysis. ChemistrySelect , 1 (10) pp. 2217-2220. 10.1002/slct.201600515, which has been published in final form at http://doi.org/10.1002/slct.201600515. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1524199 |
Archive Staff Only
 |
View Item |
