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Strategies for fast and low-dose laboratory-based phase contrast tomography for microstructural scaffold analysis in tissue engineering

Hagen, CK; Maghsoudlou, P; Totonelli, G; Diemoz, PC; Endrizzi, M; Zamir, A; Coan, A; ... Olivo, A; + view all (2016) Strategies for fast and low-dose laboratory-based phase contrast tomography for microstructural scaffold analysis in tissue engineering. In: Stock, SR and Muller, B and Wang, G, (eds.) (Proceedings) SPIE 9967, Developments in X-Ray Tomography X. (pp. p. 996705). International Society for Optical Engineering: USA. Green open access

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Abstract

The application of x-ray phase contrast computed tomography (PCT) to the field of tissue engineering is dis- cussed. Specific focus is on the edge illumination PCT method, which can be adapted to weakly coherent x-ray sources, permitting PCT imaging in standard (non-synchrotron) laboratory environments. The method was applied to a prominent research topic in tissue engineering, namely the development of effective and reliable decellularization protocols to derive scaffolds from native tissue. Results show that edge illumination PCT provides sufficient image quality to evaluate the microstructural integrity of scaffolds and, thus, to assess the performance of the used decellularization technique. In order to highlight that edge illumination PCT can ultimately comply with demands on a high specimen throughput and low doses of radiation, recently developed strategies for scan time and dose reduction are discussed.

Type: Proceedings paper
Title: Strategies for fast and low-dose laboratory-based phase contrast tomography for microstructural scaffold analysis in tissue engineering
Event: SPIE 9967, Developments in X-Ray Tomography X
Location: San Diego, California, US
Dates: 28 August 2016 - 01 September 2016
Open access status: An open access version is available from UCL Discovery
DOI: 10.1117/12.2237594
Publisher version: http://dx.doi.org/10.1117/12.2237594
Additional information: Charlotte K. Hagen ; Panagiotis Maghsoudlou ; Giorgia Totonelli ; Paul C. Diemoz ; Marco Endrizzi ; Anna Zamir ; Paola Coan ; Alberto Bravin ; Paolo De Coppi and Alessandro Olivo " Strategies for fast and low-dose laboratory-based phase contrast tomography for microstructural scaffold analysis in tissue engineering ", Proc. SPIE 9967, Developments in X-Ray Tomography X, 996705 (October 3, 2016); doi:10.1117/12.2237594; http://dx.doi.org/10.1117/12.2237594 Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Keywords: Tissue Engineering, Decellularization, X-ray phase contrast, Computed Tomography
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1520842
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