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Placental vascularity and markers of angiogenesis in relation to prenatal growth status in overnourished adolescent ewes

Carr, DJ; David, AL; Aitken, RP; Milne, JS; Borowicz, PP; Wallace, JM; Redmer, DA; (2016) Placental vascularity and markers of angiogenesis in relation to prenatal growth status in overnourished adolescent ewes. Placenta , 46 pp. 79-86. 10.1016/j.placenta.2016.08.076. Green open access

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Abstract

Introduction Placental vascularity may be important in the development of fetal growth restriction (FGR). The overnourished adolescent ewe is a robust model of the condition, with ∼50% of offspring demonstrating FGR (birthweight >2 standard deviations below optimally-fed control mean). We studied whether placental vascularity, angiogenesis and glucose transport reflect FGR severity. Methods Singleton pregnancies were established in adolescent ewes either overnourished to putatively restrict fetoplacental growth (n = 27) or control-fed (n = 12). At 131d (term = 145d) pregnancies were interrupted and fetuses classified as FGR (n = 17, <4222 g, -2SD below control-fed mean) or non-FGR (n = 10). Placentome capillary area density (CAD), number density (CND), surface density (CSD), and area per capillary (APC) in the fetal cotyledon (COT) and maternal caruncle (CAR) were analysed using immunostaining. COT/CAR mRNA expression of angiogenic ligands/receptors and glucose transporters were measured by qRT-PCR. Results Fetal weight was reduced in FGR vs. Non-FGR/Control groups. Total placentome weight was Control > Non-FGR > FGR and fetal:placental weight ratios were higher in overnourished versus Control groups. COT vascular indices were Non-FGR > FGR > Control. COT-CAD, CSD and APC were significantly greater in Non-FGR overnourished versus Control and intermediate in FGR groups. CAR vascularity did not differ. CAR-VEGFA/FLT1/KDR/ANGPT1/ANGPT2/SLC2A1/SLC2A3 mRNA was lower and COT-ANGPT2 higher in overnourished versus Control groups. Discussion Relative to control-intake pregnancy, overnourished pregnancies are characterised by higher COT vascularity, potentially a compensatory response to reduced nutrient supply, reflected by higher fetal:placental weight ratios. Compared with overnourished pregnancies where fetal growth is relatively preserved, overnourished pregnancies culminating in marked FGR have less placental vascularity, suggesting incomplete adaptation to the prenatal insult.

Type: Article
Title: Placental vascularity and markers of angiogenesis in relation to prenatal growth status in overnourished adolescent ewes
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.placenta.2016.08.076
Publisher version: http://dx.doi.org/10.1016/j.placenta.2016.08.076
Language: English
Additional information: Placental vascularity, Fetal growth restriction, Angiogenic factors, Sheep, Angiopoietin
Keywords: Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Obstetrics & Gynecology, Reproductive Biology, Placental vascularity, Fetal growth restriction, Angiogenic factors, Sheep, Angiopoietin, MESSENGER-RNA EXPRESSION, GROWING ADOLESCENT, LATE-GESTATION, FETAL-GROWTH, SHEEP, PREGNANCY, RESTRICTION, ANGIOPOIETIN-2, CONSEQUENCES, NUTRITION
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Maternal and Fetal Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/1519988
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