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Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function.

Clarkin, CE; Mahmoud, M; Liu, B; Sobamowo, EO; King, A; Arthur, H; Jones, PM; (2016) Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function. BMC Res Notes , 9 362-. 10.1186/s13104-016-2142-z. Green open access

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Abstract

BACKGROUND: Endoglin/CD105 is an auxiliary receptor for transforming growth factor-β with established roles in vascular remodelling. It has recently been shown that heterozygous endoglin deficiency in mice decreases insulin secretion in an animal model of obesity, highlighting a potential role for endoglin in the regulation of islet function. We have previously identified two different populations of endoglin expressing cells in human and mouse islets which are: (i) endothelial cells (ECs) and (ii) islet mesenchymal stromal cells. The contribution of islet EC endoglin expression to islet development and sensitivity to VEGF is unknown and is the focus of this study. RESULTS: In vitro culture of mouse islets with VEGF164 for 48 h increased endoglin mRNA levels above untreated controls but VEGF did not modulate VEGFR2, CD31 or CD34 mRNA expression or islet viability. Removal of EC-endoglin expression in vivo reduced islet EC area but had no apparent effect on islet size or architecture. CONCLUSION: EC-specific endoglin expression in islets is sensitive to VEGF and plays partial roles in driving islet vascular development, however such regulation appears to be distinct to mechanisms required to modulate islet viability and size.

Type: Article
Title: Modulation of endoglin expression in islets of langerhans by VEGF reveals a novel regulator of islet endothelial cell function.
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/s13104-016-2142-z
Keywords: Endoglin, Hypoxia inducible factor-1 alpha, Islets, VEGF, Animals, Antigens, CD34, Endoglin, Endothelial Cells, Gene Expression Regulation, Islets of Langerhans, Male, Mice, Mice, Inbred ICR, Platelet Endothelial Cell Adhesion Molecule-1, RNA, Messenger, Recombinant Proteins, Signal Transduction, Tissue Culture Techniques, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-2
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Metabolism and Experi Therapeutics
URI: https://discovery.ucl.ac.uk/id/eprint/1519655
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