Marshall, A;
Celentano, A;
Cirillo, N;
Mignogna, MD;
McCullough, M;
Porter, S;
(2016)
Antimicrobial activity and regulation of CXCL9 and CXCL10 in oral keratinocytes.
European Journal of Oral Sciences
, 124
(5)
pp. 433-439.
10.1111/eos.12293.
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Abstract
Chemokine (C-X-C motif) ligand (CXCL)9 and CXCL10 are dysregulated in oral inflammatory conditions, and it is not known if these chemokines target microorganisms that form oral biofilm. The aim of this study was to investigate the antimicrobial activity of CXCL9 and CXCL10 on oral microflora and their expression profiles in oral keratinocytes following exposure to inflammatory and infectious stimuli. Streptococcus sanguinis was used as a model and Escherichia coli as a positive control. The antimicrobial effect of CXCL9/CXCL10 was tested using a radial diffusion assay. mRNA transcripts were isolated from lipopolysaccharide (LPS)-treated and untreated (control) oral keratinocyte cell lines at 2-, 4-, 6-, and 8-h time-points of culture. The CXCL9/10 expression profile in the presence or absence of interferon-γ (IFN-γ) was assessed using semiquantitative PCR. Although both chemokines demonstrated antimicrobial activity, CXCL9 was the most effective chemokine against both S. sanguinis and E coli. mRNA for CXCL10 was expressed in control cells and its production was enhanced at all time-points following stimulation with LPS. Conversely, CXCL9 mRNA was not expressed in control or LPS-stimulated cells. Finally, stimulation with IFN-γ enhanced basal expression of both CXCL9 and CXCL10 in oral keratinocytes. Chemokines derived from oral epithelium, particularly CXCL9, demonstrate antimicrobial properties. Bacterial and inflammatory-stimulated up-regulation of CXCL9/10 could represent a key element in oral bacterial colonization homeostasis and host-defense mechanisms.
| Type: | Article |
|---|---|
| Title: | Antimicrobial activity and regulation of CXCL9 and CXCL10 in oral keratinocytes |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/eos.12293 |
| Publisher version: | http://dx.doi.org/10.1111/eos.12293 |
| Language: | English |
| Additional information: | Copyright © 2016 Eur J Oral Sci. This is the peer reviewed version of the following article: Marshall, A; Celentano, A; Cirillo, N; Mignogna, MD; McCullough, M; Porter, S; (2016) Antimicrobial activity and regulation of CXCL9 and CXCL10 in oral keratinocytes. European Journal of Oral Sciences , 124 (5) pp. 433-439. 10.1111/eos.12293, which has been published in final form at http://dx.doi.org/10.1111/eos.12293. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Keywords: | Streptococcus sanguinis, CCL28, CXCL10, CXCL9, chemokines |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1517984 |
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