Brew, O;
Nikolopoulou, E;
Hughes, A;
Christian, M;
Lee, Y;
Oduwole, O;
Sullivan, MHF;
(2016)
Quality of placental RNA: Effects of explant size and culture duration.
Placenta
, 46
pp. 45-48.
10.1016/j.placenta.2016.08.083.
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Abstract
We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.
| Type: | Article |
|---|---|
| Title: | Quality of placental RNA: Effects of explant size and culture duration |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.placenta.2016.08.083 |
| Publisher version: | http://dx.doi.org/10.1016/j.placenta.2016.08.083 |
| Language: | English |
| Additional information: | © 2016 Elsevier Ltd. All rights reserved. This manuscript version is made available under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International license (CC BY-NC-ND 4.0). This license allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licenses are available at https://creativecommons.org/licenses/. Access may be initially restricted by the publisher. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Obstetrics & Gynecology, Reproductive Biology, Placenta, RNA Quality, Micro Explant, Macro Explants, Explant Viability, Oxygen Concentration, Syncytial Regeneration, Organ-Culture, In-Vitro, Human Trophoblast, Viability, Syncytiotrophoblast, Differentiation, Regeneration, Tissue, Oxygen, Villi |
| UCL classification: | UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1516731 |
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